Recombinant Human BPGM Protein (His Tag)

Specifications

Catalog number

PKSH032119-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Bisphosphoglycerate Mutase (BPGM) is a member of the Phosphoglycerate Mutase family and BPG-Dependent PGAM subfamily. BPGM is a multifunctional enzyme. BPGM catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. It also has phosphoglycerate phosphomutase activity. BPGM plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG) . Deficiency of BPGM increases the affinity of cells for oxygen and result in hemolytic anemia.

Abbreviation

BPGM

Synonyms

2,3-Bisphosphoglycerate Mutase Erythrocyte;2,3-Bisphosphoglycerate Synthase;2,3-Diphosphoglycerate Mutase; BPG-Dependent PGAM; BPGM; Bisphosphoglycerate Mutase; DPGM

UniProt

P07738

Accession Number

P07738

Expression System

E.coli

Tag

C-His

Sequence

Ser2-Lys259

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

31.0 kDa

Observed Molecular Weight

30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, pH 8.0.

Species

Human