Recombinant Human Carbonic Anhydrase 1/CA1 Protein (His Tag)

Specifications

Catalog number

PKSH032156-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.

Abbreviation

Carbonic Anhydrase 1; CA1

Synonyms

CA-I; CA1; CAB; Carbonate Dehydratase I; Carbonic Anhydrase 1; Carbonic Anhydrase B; Carbonic Anhydrase I

UniProt

P00915

Accession Number

P00915

Expression System

E.coli

Tag

C-His

Sequence

Ala2-Phe261

Field of Research

Cell biology; Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

29.9 kDa

Observed Molecular Weight

25-35 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 12.5mM Tris-HCl, 75mM NaCl, pH 7.5.

Species

Human