Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag)

Specifications

Catalog number

PKSH032163-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide. CAs is associated with many biological processes; including calcification; respiration; bone resorption; acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart; pancreas; kidney; placenta; lung; and skeletal muscle; but it is restricted to the liver. CA5B is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA; CA-VA. CA5B is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA) ; saccharin; and Foscarnet.

Abbreviation

Carbonic Anhydrase 5B; CA5B

Synonyms

CA-VB; CA5B; Carbonate Dehydratase VB; Carbonic Anhydrase 5B Mitochondrial; Carbonic Anhydrase VB

UniProt

Q9Y2D0

Accession Number

Q9Y2D0

Expression System

E.coli

Tag

C-His

Sequence

Cys34-Pro317

Field of Research

Signal Transduction; Tags & Cell Markers; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

33.8 kDa

Observed Molecular Weight

33 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.

Species

Human