Recombinant Human Carboxylesterase 1/CES1 Protein (His Tag)

Specifications

Catalog number

PKSH032167-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carboxylesterase 1 (CES1) is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. These enzymes have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They are major determinants of the pharmacokinetic behavior of most therapeutic agents containing an ester or amide bond. CES1 shares the serine hydrolase fold observed in other esterases. CES1 hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. CES1 participates in detoxification of drugs such as cocaine and heroin in serum and liver. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds.

Abbreviation

Carboxylesterase 1; CES1

Synonyms

ACAT; Acyl-Coenzyme A:Cholesterol Acyltransferase; Brain Carboxylesterase hBr1; CE-1; Carboxylesterase 1; Cocaine Carboxylesterase; Egasyn; HMSE; Liver Carboxylesterase 1; Methylumbelliferyl-Acetate Deacetylase 1; Monocyte/Macrophage Serine Esterase; Retinyl E; hCE-1

UniProt

P23141

Accession Number

P23141-3

Expression System

HEK293 Cells

Tag

C-His

Sequence

His19-Glu562

Field of Research

Cardiovascular, Signal Transduction, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

61.1 kDa

Observed Molecular Weight

60 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0.

Species

Human