Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)

Specifications

Catalog number

PKSH032168-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E.

Abbreviation

Carboxypeptidase A1; CPA1

Synonyms

CPA; CPA1; Carboxypeptidase A1

UniProt

P15085

Accession Number

AAH05279.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Lys17-Tyr419

Field of Research

Signal Transduction; Cell biology; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

46.6 kDa

Observed Molecular Weight

44 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mm NaCl, pH 7.5.

Species

Human