Recombinant Human Carboxypeptidase B1/CPB1 Protein (His Tag)

Specifications

Catalog number

PKSH032171-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carboxypeptidase B1 is an exopeptidase which specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis and pancreatic graft rejection.Human CPB1 consists of a signal peptide, a pro region, and a mature chain. The purified rhCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro.

Abbreviation

Carboxypeptidase B1; CPB1

Synonyms

CPB; CPB1; Carboxypeptidase B; PASP; PCPB; Pancreas-Specific Protein

UniProt

P15086

Accession Number

P15086

Expression System

HEK293 Cells

Tag

C-His

Sequence

His16-Tyr417

Field of Research

Signal Transduction; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

46.9 kDa

Observed Molecular Weight

47 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, pH 5.5.

Species

Human