Recombinant Human Carboxypeptidase B2/CPB2 Protein (His Tag)

Specifications

Catalog number

PKSH032172-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carboxypeptidase B2 (CPB2) is a secreted enzyme that belongs to the peptidase M14 family. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen by the liver and circulates in the plasma as a plasminogen-bound zymogen. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides, such as kinins or anaphylatoxins, in the circulation regulating their activities. CPB2 also down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. CPB2 exhibits carboxypeptidase activity when it is activated by proteolysis at residue Arg92 of the thrombin/thrombomodulin complex. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Abbreviation

Carboxypeptidase B2; CPB2

Synonyms

CPB2; CPU; Carboxypeptidase B2; Carboxypeptidase U; Plasma Carboxypeptidase B; TAFI; Thrombin-Activable Fibrinolysis Inhibitor; pCPB

UniProt

Q96IY4

Accession Number

NP_001863.3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Phe23-Val423

Field of Research

Signal Transduction; Cardiovascular; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

47.0 kDa

Observed Molecular Weight

55-65 kDa

Formulation

2

Species

Human