Recombinant Human Caspase-10/CASP10 Protein (His Tag)

Specifications

Catalog number

PKSH032176-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Caspase-10 (CASP10) is a 521 amino acid protein member of the Cysteine-Aspartic Acid Protease (Caspase) family. CASP10 contains two DED (Death Effector) domains and is detectable in most tissues. CASP10 cleavage by Granzyme B and autocatalytic activity generate the two active subunits: Caspase-10 subunit p23/17, Caspase-10 subunit p12. Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the execution-phase of cell apoptosis, the initiation and execution. Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7) . CASP10 cleaves and activates caspases 3 and 7, but itself is processed by caspase 8. Defects in CASP10 are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome.

Abbreviation

Caspase-10; CASP10

Synonyms

Apoptotic Protease Mch-4; CASP-10; CASP10; Caspase-10; FAS-Associated Death Domain Protein Interleukin-1B-Converting Enzyme 2; FLICE2; ICE-Like Apoptotic Protease 4; MCH4

UniProt

Q92851

Accession Number

Q92851-4

Expression System

E.coli

Tag

C-His

Sequence

Val220-Ile480

Field of Research

Cell Biology, Cancer, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 70 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

30.1 kDa

Observed Molecular Weight

33 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 1mM DTT, 0.05% Tween 80, pH8.5.

Species

Human