Recombinant Human CCL16 Protein

Specifications

Catalog number

PKSH032188-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CCL16 is a member of CC chemokine family. CCL16 cDNA encodes a 120 amino acid peptide along with a 23 amino acids signal peptide that is cleaved to generate 97 amino acid protein. CCL16 is distantly related to other CC chemokines, showing less than 30% sequence identity. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8. Recombinant CCL16 has been shown to chemoattract human monocytes and THP1 cells but not resting lymphocytes nor neutrophils. CCL16 has potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. CCL16ninduces a calcium flux in THP1 cells that can be desensitized by prior exposure to RANTES, suggesting that CCL16 and RANTES share the same receptor in THP1 cells.

Abbreviation

CCL16

Synonyms

C-C Motif Chemokine 16; CCL16; Chemokine CC-4; Chemokine LEC; HCC-4; IL-10-Inducible Chemokine; ILINCK; LCC-1; LMC; Liver-Expressed Chemokine; Lymphocyte and Monocyte Chemoattractant; MTN-1; Monotactin-1; NCC-4; NCC4; SCYA16; Small-Inducible Cytokine A16

UniProt

O15467

Accession Number

O15467

Expression System

E.coli

Tag

None

Sequence

Gln24-Gln120

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

11.0 kDa

Observed Molecular Weight

14 kDa

Species

Human