Recombinant Human CD19/Leu-12 Protein (Fc Tag)

Specifications

Catalog number

PKSH032206-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD19 is a single-pass type I membrane protein containing 2 Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact; it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation; the cytoplasmic tail of CD19 becomes phosphorylated; which leads to binding by Src-family kinases and recruitment of PI-3 kinase. CD19 Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) which is a primary immunodeficiency characterized by antibody deficiency; hypogammaglobulinemia; recurrent bacterial infections and an inability to mount an antibody response to antigen.

Abbreviation

CD19; Leu-12

Synonyms

B-Lymphocyte Antigen CD19; B-Lymphocyte Surface Antigen B4; CD19; CVID3; Differentiation Antigen CD19; T-Cell Surface Antigen Leu-12

UniProt

P15391

Accession Number

P15391

Expression System

CHO Stable Cells

Tag

C-Fc

Sequence

Pro20-Lys291

Applications

ELISA

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.3 kDa

Observed Molecular Weight

80-95 kDa

Species

Human