Recombinant Human CD40L/TNFSF5 Protein (Human Cells)

Specifications

Catalog number

PKSH032214-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members; CD40LG exists as a trimer in membrane bound and soluble form; both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma; TNF-alpha; and IL2.

Abbreviation

CD40L; TNFSF5

Synonyms

CD154; CD40 Ligand; CD40-L; CD40L; CD40LG; HIGM1; IGM; IMD3; T-BAM; T-Cell Antigen Gp39; TNF-Related Activation Protein; TNFSF5; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; hCD40L

UniProt

P29965

Accession Number

P29965

Expression System

HEK293 Cells

Tag

None

Sequence

Met 113-Leu261

Applications

BLI

Field of Research

Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Human CD40-Fc on Protein A Biosensor, can bind Human CD40L with an affinity constant of 3.29 nM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

16.2 kDa

Observed Molecular Weight

16-18 kDa

Species

Human