Recombinant Human PRKG1 Protein (His Tag)

Specifications

Catalog number

PKSH032242-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CGMP-Dependent Protein Kinase 1 (PRKG1) belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. PRKG1 contains one AGC-kinase C-terminal domain, two cyclic nucleotide-binding domains, and one protein kinase domain. PRKG1 is mainly expressed in the lung and placenta. PRKG1 acts as a key mediator of the nitric oxide (NO) /cGMP signaling pathway. PRKG1 can phosphorylate many proteins that regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm, and nociception.

Abbreviation

PRKG1

Synonyms

PRKG1; PRKG1B; PRKGR1A; PRKGR1B; cGK 1; cGK1; cGKI; cGMP-Dependent Protein Kinase 1; cGMP-Dependent Protein Kinase I

UniProt

Q13976

Accession Number

Q13976-2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gly2-Pro686

Field of Research

Cardiovascular, Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

78.8 kDa

Observed Molecular Weight

75 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH8.0.

Species

Human