Recombinant Human Chitotriosidase/CHIT1 Protein (His Tag)

Specifications

Catalog number

PKSH032245-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chitotriosidase-1 (CHIT1) is a glycoprotein that belongs to the Glycosyl Hydrolase 18 family and Chitinase class II subfamily. It is secreted by cultured macrophages and serves to degrade chitin, chitotriose and chitobiose. CHIT1 may participate in the defense against nematodes and other pathogens. It is highly expressed in the plasma of patients with Gaucher's disease type I, which can be used as diagnostic aid and to evaluate the success of treatment that brings levels back to normal. The amino acid sequence of human CHIT1 is 99%, 76%, 75% and 55% identical to that of chimpanzee, rat, mouse and fruit fly, respectively.

Abbreviation

Chitotriosidase; CHIT1

Synonyms

CHI3; CHIT; CHIT1; CHITD; Chitinase-1; Chitotriosidase-1

UniProt

Q13231

Accession Number

AAI03696.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala22-Asn466

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

50.4 kDa

Observed Molecular Weight

54 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.

Species

Human