Recombinant Human Coagulation Factor IX/F9 Protein (His Tag)

Specifications

Catalog number

PKSH032264-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Coagulation factor IX (F9), is a member of the peptidase S1 family. It contains two EGF-like domains, a Gla domain and a peptidase S1 domain. It is primarily expressed in the liver and secreted in plasma. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ ions, phospholipids, and factor VIIIa. Mutations in position 43 and 46 prevents cleavage of the propeptide, mutation in position 93 probably fails to bind to cell membranes, mutation in position 191 or in position 226 prevent cleavage of the activation peptide. Mutations of human F9 can result in thrombophilia and recessive X-linked hemophilia B (HEMB) . An X-linked blood coagulation disorder characterized by a permanent tendency to hemorrhage, due to factor IX deficiency. It is phenotypically similar to hemophilia A, but patients present with fewer symptoms. Many patients are asymptomatic until the hemostatic system is stressed by surgery or trauma.

Abbreviation

Coagulation Factor IX; F9

Synonyms

Christmas factor; Coagulation factor 9; Coagulation factor IX; Coagulation factor IXa heavy chain; Coagulation factor IXa light chain; F9; FIX; HEMB; P19; PTC; Plasma thromboplastin component; THPH8

UniProt

P00740

Accession Number

P00740

Expression System

HEK293 Cells

Tag

C-His

Sequence

Thr 29-Thr461

Field of Research

Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

49.8 kDa

Observed Molecular Weight

60-90 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.

Species

Human