Recombinant Human CRHBP Protein (His Tag)

Specifications

Catalog number

PKSH032283-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Corticotropin-Releasing Factor-Binding Protein (CRHBP) is a 37 kDa secreted glycoprotein that binds both CRH and urocortin in a 42 kDa extracellular complex. The molecule is approximately 300 amino acids in length and demonstrates five intrachain disulfide bonds. Difference between CRHBP from different species exist, human CRHBP is found in plasma while rodent and sheep CRHBP is limited to neuroendocrine tissues. CRHBP may inactivate CRH and may prevent inappropriate pituitary-adrenal stimulation in pregnancy. CRHBP is presumed to either sequester CRH, rendering it unavailable to cells or transport it to target tissues. Although CRF-BP concentration in the human peripheral circulation is normally low, it increases throughout pregnancy and fall back rapidly after parturition.

Abbreviation

CRHBP

Synonyms

CRF-BP; CRF-Binding Protein; CRFBP; CRH-BP; CRHBP; Corticotropin-Releasing Factor-Binding Protein; Corticotropin-Releasing Hormone-Binding Protein

UniProt

P24387

Accession Number

P24387

Expression System

HEK293 Cells

Tag

C-His

Sequence

Tyr25-Leu322

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.4 kDa

Observed Molecular Weight

37 kDa

Species

Human