Recombinant Human Creatine Kinase, Muscle/CKM Protein (His Tag)

Specifications

Catalog number

PKSH032285-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Creatine kinase M-type is also known as Creatine kinase M chain, M-CK. It is a protein that in humans is encoded by the CKM gene. It belongs to the ATP:guanido phosphotransferase family, containing 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. Creatine kinase M-type can reversibly catalyzes the transfer of phosphate between ATP and various phosphogens. It plays a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Abbreviation

CKM

Synonyms

CKM; CKMM; Creatine kinase M chain; Creatine kinase M-type; M-CK

UniProt

P06732

Accession Number

AAP35439.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Lys381

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

44.1 kDa

Observed Molecular Weight

46 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.

Species

Human