Recombinant Human CXCL7/NAP-2 Protein

Specifications

Catalog number

PKSH032305-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Chemokine (C-X-C motif) Ligand 7 (CXCL7) ; also known as neutrophil activating peptide 2 (NAP-2) ; is a member of the CXC chemokines containing an ELR domain (Glu-Leu-Arg tripeptide motif) . Similar to other ELR domain containing CXC chemokines; such as IL-8 and the GRO proteins; CXCL7 binds CXCR2; chemoattracts and activates neutrophils. CXCL7; Connective Tissue Activating Protein III (CTAPIII) and βthrombogulin (βTG) ; are proteolytically processed carboxylterminal fragments of platelet basic protein (PBP) which is found in the alphagranules of human platelets. Although CTAPIII; βTG; and PBP represent amino-terminal extended variants of NAP2 and possess the same CXC chemokine domains; these proteins do not exhibit CXCL7/NAP2 activity. CXCL7 induces cell migration through the G-protein-linked receptor CXCR-2.

Abbreviation

CXCL7; NAP-2

Synonyms

C-X-C Motif Chemokine 7; CTAP3; CXCL7; LDGF; Leukocyte-Derived Growth Factor; MDGFSmall-Inducible Cytokine B7; Macrophage-Derived Growth Factor; PBP; PPBP; Platelet Basic Protein; SCYB7; TGB1; THBGB1

UniProt

P02775

Accession Number

P02775

Expression System

E.coli

Tag

N-His

Sequence

Ala59-Asp128

Applications

Cell culture

Field of Research

Signal Transduction; Cardiovascular; immunology

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

8.4 kDa

Observed Molecular Weight

11 kDa

Species

Human