Recombinant Human Cyclophilin C/PPIC Protein (His&Trx Tag)

Specifications

Catalog number

PKSH032316-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cyclophilin C is an enzyme (EC 5.2.1.8) found in both prokaryotes and eukaryotes that interconverts the cis and trans isomers of peptide bonds with the amino acid proline. Proline has an unusually conformationally restrained peptide bond due to its cyclic structure with its side chain bonded to its secondary amine nitrogen. Most amino acids have a strong energetic preference for the trans peptide bond conformation due to steric hindrance, but prolines unusual structure stabilizes the cis form so that both isomers are populated under biologically relevant conditions. Proteins with prolyl isomerase activity include cyclophilin, FKBPs, and parvulin, although larger proteins can also contain prolyl isomerase domains.

Abbreviation

Cyclophilin C; PPIC

Synonyms

CYPC; Cyclophilin C; PPIC; PPIase C; Peptidyl-Prolyl Cis-Trans Isomerase C; Rotamase C

UniProt

P45877

Accession Number

P45877

Expression System

E.coli

Tag

N-Trx-His

Sequence

Lys31-Asp182

Field of Research

Signal Transduction; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

33.8 kDa

Observed Molecular Weight

34 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.

Species

Human