Recombinant Human Cytochrome C/CYCS Protein (His Tag)

Specifications

Catalog number

PKSH032336-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytochrome C (CYCS) is a small heme protein that belongs to the cytochrome c family. It is found loosely associated with the inner membrane of the mitochondrion. Cytochrome C is a highly soluble protein that functions as a central component of the electron transport chain in mitochondria. CYCS transfers electrons between Complexes III (Coenzyme Q - Cyt C reductase) and IV (Cyt C oxidase) . CYCS plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of Cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Abbreviation

Cytochrome C; CYCS

Synonyms

CYC; CYCS; Cytochrome C

UniProt

P99999

Accession Number

P99999

Expression System

E.coli

Tag

C-His

Sequence

Gly2-Glu105

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

12.8 kDa

Observed Molecular Weight

16 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 200mM NaCl, 50% Glycerol, 0.05% Tween 80, pH7.0.

Species

Human