Recombinant Human NCK1 Protein (His Tag)

Specifications

Catalog number

PKSH032339-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.

Abbreviation

NCK1

Synonyms

Cytoplasmic Protein NCK1; NCK; NCK adaptor Protein 1; NCK1; Nck-1; SH2/SH3 Adaptor Protein NCK-Alpha

UniProt

P16333

Accession Number

P16333

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Ser377

Field of Research

Signal Transduction; Cancer; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.0 kDa

Observed Molecular Weight

50 kDa

Species

Human