Recombinant Human D-Amino-Acid Oxidase/DAO Protein (His Tag)

Specifications

Catalog number

PKSH032342-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups.

Abbreviation

DAO

Synonyms

D-Amino-Acid Oxidase; DAAO; DAMOX; DAO

UniProt

P14920

Accession Number

P14920

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Leu347

Field of Research

Signal Transduction; Tags & Cell Markers; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

41.6 kDa

Observed Molecular Weight

40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 20% Glycerol, 1mM DTT, 2mM EDTA, pH 8.0.

Species

Human