Recombinant Human Deoxycytidine Kinase/DCK Protein (His &T7 Tag)

Specifications

Catalog number

PKSH032348-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Deoxycytidine Kinase (DCK) is a member of the DCK/DGK family. DCK exists as a homodimer and is localized to the nucleus. DCK is required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG), and deoxyadenosine (dA) . DCK has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. In addition, DCK is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. DCK is clinically important because of its relationship to drug resistance and sensitivity.

Abbreviation

Deoxycytidine Kinase; DCK

Synonyms

DCK; Deoxycytidine Kinase; dCK

UniProt

P27707

Accession Number

P27707

Expression System

E.coli

Tag

N-His-T7

Sequence

Met 1-Leu260

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

34.0 kDa

Observed Molecular Weight

30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, 1mM TCEP, pH 7.4.

Species

Human