Recombinant Human Diamine Oxidase/AOC1 Protein (His Tag)

Specifications

Catalog number

PKSH032352-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Amiloride-sensitive amine oxidase (AOC1) belongs to the copper/topaquinone oxidase family. The protein exists as homodimer by disulfide and mainly located in placenta and kidney. AOC1 catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. The activity of this protein can be inhibited by amiloride in a competitive manner. It is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels.

Abbreviation

Diamine Oxidase; AOC1

Synonyms

ABP1; AOC1; Amiloride-binding protein 1; Amiloride-sensitive amine oxidase [copper-containing]; Amine oxidase copper domain-containing protein 1; DAO; DAO1; Diamine oxidase; Histaminase; KAO; Kidney amine oxidase

UniProt

P19801

Accession Number

AAH14093.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu20-Val751

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

84.4 kDa

Observed Molecular Weight

90 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.

Species

Human