Recombinant Human DLG4 Protein (His Tag)

Specifications

Catalog number

PKSH032358-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Disks large homolog 4 (DLG4) is a cell membrane protein and it is a member of the membrane-associated guanylate kinase (MAGUK) family. The protein contains 1 guanylate kinase-like domain,3 PDZ (DHR) domains and 1 SH3 domain. With PSD-93 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. DLG4 is the best studied member of the MAGUK-family of PDZ domain-containing proteins. Like all MAGUK-family proteins, its basic structure includes three PDZ domains, an SH3 domain, and a guanylate kinase-like domain (GK) connected by disordered linker regions. It is almost exclusively located in the post synaptic density of neurons, and is involved in anchoring synaptic proteins. Its direct and indirect binding partners include neuroligin, NMDA receptors, AMPA receptors, and potassium channels.

Abbreviation

DLG4

Synonyms

DLG4; Disks large homolog 4; PSD-95; PSD95; Postsynaptic density protein 95; SAP-90; SAP90; Synapse-associated protein 90

UniProt

P31016

Accession Number

NP_062567

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Leu724

Field of Research

Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 77 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

81.9 kDa

Observed Molecular Weight

110 kDa

Species

Human