Recombinant Human DFFA Protein (His Tag)

Specifications

Catalog number

PKSH032359-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

DNA Fragmentation Factor Subunit Alpha (DFFA) . DFFA exists as a heterodimer (DFF) with DFFB. DFF is activated once DFFA is cleaved by Caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.

Abbreviation

DFFA

Synonyms

DFF-45; DFF1; DFF45; DFFA; DNA Fragmentation Factor 45 kDa Subunit; DNA Fragmentation Factor Subunit Alpha; H13; ICAD; Inhibitor of CAD

UniProt

O00273

Accession Number

AAH07721.1

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Thr331

Field of Research

Cell biology; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

38.7 kDa

Observed Molecular Weight

40-50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

Human