Recombinant Human STUB1 Protein

Specifications

Catalog number

PKSH032367-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

E3 Ubiquitin-Protein Ligase CHIP is a cytoplasmic protein. CHIP is highly expressed in skeletal muscle, heart, pancreas, brain and placenta. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain; lead to in client substrate ubiquitylation and degradation by the proteasome. CHIP targets misfolded chaperone substrates towards proteasomal degradation. CHIP mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. CHIP plays a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. It also may regulate the receptor stability and activity through proteasomal degradation.

Abbreviation

STUB1

Synonyms

Antigen NY-CO-7; CHIP; CLL-Associated Antigen KW-8; Carboxy Terminus of Hsp70-Interacting Protein; E3 Ubiquitin-Protein Ligase CHIP; STIP1 Homology and U Box-Containing Protein 1; STUB1

UniProt

Q9UNE7

Accession Number

Q9UNE7

Expression System

E.coli

Tag

None

Sequence

Met 1-Tyr303

Field of Research

Signal Transduction; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

34.9 kDa

Observed Molecular Weight

33 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

Human