Recombinant Human PI3/Elafin Protein (His Tag)

Specifications

Catalog number

PKSH032375-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Elafin is a secreted protein. Elafin consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize Elafin covalently to specific sites on extracellular matrix proteins. It is shown that Elafin can be used as a biomarker for graft versus host disease of the skin. Elafin is expressed in the skin during wound healing through activation of the epidermal growth factor receptor. Elafin may prevent elastase-mediated tissue proteolysis.

Abbreviation

PI3; Elafin

Synonyms

ESI; Elafin; Elastase-Specific Inhibitor; PI-3; PI3; Peptidase Inhibitor 3; Protease inhibitor WAP3; SKALP; Skin-Derived Antileukoproteinase; WAP Four-Disulfide Core Domain Protein 14; WAP3; WFDC14

UniProt

P19957

Accession Number

P19957

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala23-Gln117

Field of Research

Microbiology; Cell biology; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

11.0 kDa

Observed Molecular Weight

13-18 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.

Species

Human