Recombinant Human EpCAM/TROP-1 Protein (Fc Tag)

Specifications

Catalog number

PKSH032383-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that belongs to the EPCAM family. EpCAM is composed of an extracellular domain with one thyroglobulin type-1 domain; a transmembrane domain and a cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma; but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium as an immunological barrier providing the first line of defense against infection. Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5) . EpCAM plays a role in embryonic stem cells proliferation and differentiation; it up-regulates the expression of FABP5; MYC and Cyclin A and Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells.

Abbreviation

EpCAM; TROP-1

Synonyms

Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; EGP314; Ep-CAM; Epithelial Cell Adhesion Molecule; Epithelial Cell Surface Antigen; Epithelial Glycoprotein 314; Major Gastrointestinal Tumor-Associated Protein GA733-2; Tumor-Associated Calcium

UniProt

P16422

Accession Number

AAH14785.1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gln24-Lys265

Field of Research

Tags & Cell Markers

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

54.5 kDa

Observed Molecular Weight

60-80 kDa

Species

Human