Recombinant Human EphA2 Protein (Fc Tag)

Specifications

Catalog number

PKSH032384-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin type-A receptor 2/EphA2 is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1; 2; 3; 4; and 5. A and B class Eph proteins have a common structural organization. Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells; leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. EphA2 becomes autophosphorylated following ligand binding and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways. Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin; and can be induced by p53 family transcription factors. EphA2 is upregulated in breast; prostate; and colon cancer vascular endothelium. Its ligand; EphrinA1; is expressed by the local tumor cells. In some cases; EphA2 and EphrinA1 are expressed on the same blood vessels. EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration.

Abbreviation

EphA2

Synonyms

ARCC2; CTPA; CTPP1; CTRCT6; ECK; EPHA2; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK

UniProt

P29317

Accession Number

P29317

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Ala24-Asn534

Field of Research

Signal Transduction; Cardiovascular; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

83.0 kDa

Observed Molecular Weight

90-120 kDa

Species

Human