Recombinant Human Ephrin-B1/EFNB1 Protein (His Tag)

Specifications

Catalog number

PKSH032394-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ephrin-B1, also named EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, is a single-pass type I membrane protein. It contains 1 ephrin RBD (ephrin receptor-binding) domain and belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4. The extracellular domains of human and mouse ephrin-B1 share 94% amino acid identity.

Abbreviation

Ephrin-B1; EFNB1

Synonyms

CFND; CFNS; EFB1; EFL-3; EFL3; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; EPLG2; Elk-L; Ephrin-B1; LERK-2; LERK2

UniProt

P98172

Accession Number

P98172

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu28-Gly232

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Neuroscience; Developmental Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.4 kDa

Observed Molecular Weight

28-34 kDa

Species

Human