Recombinant Human EGFR/ErbB1 Protein (aa 25-645, Fc Tag)

Specifications

Catalog number

PKSH032398-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK; PI3 kinase-AKT; PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16; activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling.

Abbreviation

EGFR; ErbB1

Synonyms

EGFR; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptortyrosine-protein kinase erbB-1

UniProt

P00533

Accession Number

P00533

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Leu25-Ser645

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

95.7 kDa

Observed Molecular Weight

120-160 kDa

Species

Human