Recombinant Human ERO1A/ERO1L Protein (His Tag)

Specifications

Catalog number

PKSH032401-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ERO1-Like Protein α (ERO1L) is an enzyme that belongs to the EROs family. ERO1L is expressed at high level in esophagus and upper digestive tract. ERO1L is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1L acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It associates with ERP44, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. ERO1L may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell. ERO1L responses to temperature stimulus, protein thiol-disulfide exchange, protein folding with or without chaperone cofactor and transport.

Abbreviation

ERO1A; ERO1L

Synonyms

ERO1-L; ERO1-L-Alpha; ERO1-Like Protein Alpha; ERO1L; Endoplasmic Oxidoreductin-1-Like Protein; Oxidoreductin-1-L-Alpha

UniProt

Q96HE7

Accession Number

Q96HE7

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu24-His468

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

53.0 kDa

Observed Molecular Weight

71 kDa

Species

Human