Recombinant Human SELENOI/EPT1 Protein (GST Tag)

Specifications

Catalog number

PKSH032407-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ethanolaminephosphotransferase 1 (EPT1) is an enzyme that belongs to the CDP-Alcohol Phosphatidyltransferase Class-I Family. EPT1 is a Selenoprotein, which contains a Selenocysteine (Sec) residue at its active site. The Selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. EPT1 catalyzes Phosphatidylethanolamine biosynthesis from CDP-Ethanolamine. It plays a central role in the formation and maintenance of vesicular membranes. EPT1 is involved in the formation of Phosphatidylethanolamine via the 'Kennedy' pathway.

Abbreviation

SELENOI; EPT1

Synonyms

EPT1; Ethanolaminephosphotransferase 1; KIAA1724; SELI; SelI; Selenoprotein I; hEPT1

UniProt

Q9C0D9

Accession Number

Q9C0D9

Expression System

E.coli

Tag

N-GST

Sequence

Met 1-Pro50

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

32.6 kDa

Observed Molecular Weight

29 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.0.

Species

Human