Recombinant Human CD32a/FCGR2A Protein (H131, His Tag)

Specifications

Catalog number

PKSH032418-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human FcγRs are divided into three classes designated FcγRI (CD64) ; FcγRII (CD32) ; and FcγRIII (CD16) ; which generate multiple isoforms; are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses; whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.Human CD32; also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a) ; FcγRII A or FCGR2A Protein; is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit; FcRγ; CD32a (FcγRII A) delivers an activating signal upon ligand binding; and results in the initiation of inflammatory responses including cytolysis; phagocytosis; degranulation; and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B; and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

Abbreviation

CD32a; FCGR2A

Synonyms

CD32; CD32A; CDw32; FCG2; FCGR2 ; FCGR2A; FCGR2A1; Fc gamma RIIA; Fc-gamma RII-a; Fc-gamma-RIIa; FcGR; FcRII-a; IGFR2; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt

P12318

Accession Number

P12318

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala36-Ile218

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.1 kDa

Observed Molecular Weight

25-32 kDa

Species

Human