Recombinant Human CD16a/FCGR3A Protein (His Tag)

Specifications

Catalog number

PKSH032419-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Receptors for the Fc region of immunoglobin G (FcγR) are divided into three classes and FcγRIII is a multifunctional; low/intermediate affinity receptor. In humans; FcγRIII is expressed as two distinct forms (FcγRIIIA and FcγRIIIB) that are encoded by two different but highly homologous genes in a cell type-specific manner. FcγRIIIB is a low-affinity; GPI-linked receptor expressed by neutrophils and eosinophils; whereas FcγRIIIA is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes; natural killer (NK) cells; monocytes; and macrophages. The FcγRIIIA receptor is involved in phagocytosis; secretion of enzymes; inflammatory mediators; antibody-dependent cellular cytotoxicity (ADCC) ; mast cell degranulation; and clearance of immune complexes. FcγRIIIA has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and delivers an activation signal in the immune responses. Aberrant expression or mutations in this gene is implicated in susceptibility to recurrent viral infections; systemic lupus erythematosus; and alloimmune neonatal neutropenia. In humans; it is a 50 -70 kD type I transmembrane activating receptor. The FcγRIIIA cDNA encodes 254 amino acid including a 16aa signal sequence; 191 amino acid ECD with two C2-type Ig-like domains; five potential N-glycosylation sites; a 22 amino acid transmembrane sequence and a 25 amino acid cytoplasmic domain.

Abbreviation

CD16a; FCGR3A

Synonyms

CD16; CD16A; CD16a; CD16a Antigen; FCG3; FCGR3; FCGR3A; Fc-Gamma RIII; Fc-Gamma RIII-Alpha; Fc-gamma RIIIa; FcR-10; FcRIII; FcRIIIa; IGFR3; IgG Fc Receptor III-2; Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A

UniProt

P08637

Accession Number

AAH17865.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gly17-Gln208

Applications

BLI

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Human IgG1 Fc on Protein-A Biosensor, can bind Human CD16a-His with an affinity constant of 0.571 uM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.7 kDa

Observed Molecular Weight

35-50 kDa

Species

Human