Recombinant Human Fibronectin/FN Protein

Specifications

Catalog number

PKSH032450-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fibronectin1 (FN1) is a secreted protein and contains 12 fibronectin type-I domains; fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II; and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.

Abbreviation

Fibronectin; FN

Synonyms

CIG; ED-B; FINC; FN; FN1; FNZ; Fibronectin; GFND; GFND2; LETS; MSF

UniProt

P02751

Accession Number

P02751

Expression System

E.coli

Tag

None

Sequence

Pro1270-Ser1546&Ala1721-Thr2016

Field of Research

Signal Transduction; Cardiovascular; Cancer; Developmental Biology; Stem cells

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

62.7 kDa

Observed Molecular Weight

60-80 kDa

Species

Human