Recombinant Human PFKFB1 Protein (His Tag)

Specifications

Catalog number

PKSH032459-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. It belongs to the phosphoglycerate mutase family. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis.

Abbreviation

PFKFB1

Synonyms

6-P2ase liver isozyme;6-bisphosphatase;6-bisphosphatase 1;6-phosphofructo-2-kinase/fructose-2;6PF-2-K/Fru-2; F6PK; Fructose-2; PFKFB1; PFRX

UniProt

P16118

Accession Number

P16118

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ser2-Tyr471

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

55.6 kDa

Observed Molecular Weight

60 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 1mM EDTA, pH 7.8.

Species

Human