Recombinant Human Galectin-3/LGALS3 Protein

Specifications

Catalog number

PKSH032474-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Galectin family of proteins consists of beta-galactoside binding lectins containing homologous carbohydrate recognition domains (CRDs) . They also possess hemagglutination activity; which is attributable to their bivalent carbohydrate binding properties. Galectins are active both intracellularly and extracellularly. They have diverse effects on many cellular functions including adhesion; migration; polarity; chemotaxis; proliferation; apoptosis; and differentiation. Galectins may therefore play a key role in many pathological states; including autoimmune diseases; allergic reactions; inflammation; tumor cell metastasis; atherosclerosis; and diabetic complications. The galectins have been classified into the prototype galectins (1; 2; 5; 7; 10; 11; 13; 14) ; which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin3) containing one CRD linked to a nonlectin domain; and the tandem repeat Galectins (4; 6; 8; 9; 12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However; via one or more as yet unidentified nonclassical secretory pathways; galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell surface glycoproteins.

Abbreviation

Galectin-3; LGALS3

Synonyms

35 kDa Lectin; CBP35; Carbohydrate-Binding Protein 35; GAL3; GALBP; GALIG; Gal-3; Galactose-Specific Lectin 3; Galactoside-Binding Protein; Galectin-3; IgE-Binding Protein; L-31; L31; LGALS2; LGALS3; Laminin-Binding Protein; Lectin L-29; MAC2; Mac-2 Antigen; P35

UniProt

P17931

Accession Number

P17931

Expression System

E.coli

Tag

None

Sequence

Ala2-Ile250

Field of Research

Cell biology; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.0 kDa

Observed Molecular Weight

30 kDa

Species

Human