Recombinant Human GDNF Protein

Specifications

Catalog number

PKSH032488-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a disulfide-linked homodimeric glycoprotein that belongs to the TGF-β superfamily. It has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. Mutations in this gene may be associated with Hirschsprung disease.

Abbreviation

GDNF

Synonyms

ATF; Astrocyte-Derived Trophic Factor; GDNF; Glial Cell Line-Derived Neurotrophic Factor; hGDNF

UniProt

P39905

Accession Number

P39905

Expression System

E.coli

Tag

C-His

Sequence

Ser78-Ile211

Applications

Cell culture

Field of Research

Neuroscience; Developmental Biology

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

16.0 kDa

Observed Molecular Weight

16 kDa

Species

Human