Recombinant Human GCDH Protein (His Tag)

Specifications

Catalog number

PKSH032495-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Glutaryl-CoA Dehydrogenase Mitochondrial (GCDH) is an enzyme that acts upon glutaryl-coenzyme A, creating crotonyl-coenzyme A. It plays a role in the metabolism of lysine, hydroxylysine and tryptophan. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive Glutaryl-CoA and electron-transfer flavoprotein to (E) -but-2-enoyl-CoA, CO2 and reduced electron-transfer flavoprotein. A defect in this enzyme is associated with neurological condition glutaric acidemia type 1 and cause a progressive form of early-onset generalized dystonia.

Abbreviation

GCDH

Synonyms

GCD; GCDH; Glutaryl-CoA Dehydrogenase Mitochondrial

UniProt

Q92947

Accession Number

Q92947

Expression System

E.coli

Tag

N-His

Sequence

Arg45-Lys438

Field of Research

Signal Transduction; Tags & Cell Markers; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

45.0 kDa

Observed Molecular Weight

41 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH 7.4.

Species

Human