Recombinant Human GRB2 Protein (His Tag)

Specifications

Catalog number

PKSH032512-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

As an adaptor protein, Growth Factor Receptor-Bound Protein 2 (GRB2) is involved in siganl transduction and consists of a central SH2 domain flanked by two SH3 domains. GRB2 associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathway.It also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK. probably via the concerted action of both its SH2 and SH3 domains.

Abbreviation

GRB2

Synonyms

ASH; Adapter Protein GRB2; GRB2; Growth Factor Receptor-Bound Protein 2; Protein Ash; SH2/SH3 Adapter GRB2

UniProt

P62993

Accession Number

P62993

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Val217

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.3 kDa

Observed Molecular Weight

25-30 kDa

Species

Human