Recombinant Human GAMT Protein (His Tag)

Specifications

Catalog number

PKSH032515-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

GAMT is a methyltransferase which belongs to the class I-like SAM-binding methyltransferase superfamily. It contains one RMT2 (arginine N-methyltransferase 2-like) domain and is expressed in liver. GAMT converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in GAMT are the cause of guanidinoacetate methyltransferase deficiency, which is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, intractable seizures and movement disturbances, severe depletion of creatine/phosphocreatine in the brain, and accumulation of guanidinoacetic acid in brain and body fluids.

Abbreviation

GAMT

Synonyms

GAMT; Guanidinoacetate N-methyltransferase; PIG2; TP53I2

UniProt

Q14353

Accession Number

Q14353

Expression System

E.coli

Tag

N-His & C-His

Sequence

Met 1-Gly236

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

29.5 kDa

Observed Molecular Weight

27-32 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, pH 8.0.

Species

Human