Recombinant Human RIDA/HRSP12 Protein (His Tag)

Specifications

Catalog number

PKSH032526-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heat-Responsive Protein 12 (HRSP12) is an endoribonuclease that belongs to the Rut family. HRSP12 is found mainly in the human adult kidney and liver and is responsible for inhibiting protein translation by cleaving mRNA. HRSP12 only cleaves phosphodiester bonds in single-stranded RNA and inhibits cell-free protein synthesis. The levels of both mRNA and protein are markedly reduced in heptatocellular tumors and in human hepatoma cell lines compared with normal liver tissues. Moreover the levels of HRSP12 are different depending on the grade of the tumor. This had led to the suggestion that HRSP12 may be an important biomarker for heptatic carcinoma.

Abbreviation

RIDA; HRSP12

Synonyms

14.5 kDa Translational Inhibitor Protein; HRSP12; Heat-Responsive Protein 12; PSP; Ribonuclease UK114; UK114 Antigen Homolog; p14.5

UniProt

P52758

Accession Number

P52758

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Leu137

Field of Research

Epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

16.7 kDa

Observed Molecular Weight

16 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0.

Species

Human