Recombinant Human HBEGF Protein (His Tag)

Specifications

Catalog number

PKSH032533-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heparin-binding EGF-like growth factor (HB-EGF) is a 12-16 kDa member of the epidermal growth factor (EGF) family. It possesses an EGF-like domain, and a heparin-binding motif. Mature HB-EGF is a soluble peptide that arises from proteolytic processing of the transmembrane form. Human HB-EGF shows 76% and 73% aa sequence identity with rat and mouse HB-EGF, respectively. It is required for normal cardiac valve formation and normal heart function, promotes smooth muscle cell proliferation. It may be involved in macrophage-mediated cellular proliferation; it is mitogenic for fibroblasts, but not endothelial cells. HB-EGF classified as a group 2 ErbB ligand based on its ability to activate both the EGF/ErbB1 and ErbB4 receptors. Activity associated with ErbB4 binding appears to be limited to non-mitogenic actions, while EGFR binding induces both mitogenic and non-mitogenic activity.

Abbreviation

HBEGF

Synonyms

DTR; DTS; DTSF; Diphtheria toxin receptor; HB-EGF; HEGFL; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; pro HB-EGF; proheparin-binding EGF-like growth factor

UniProt

Q99075

Accession Number

Q99075

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu20-Leu148

Field of Research

Signal Transduction; Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.1 kDa

Observed Molecular Weight

16-27 kDa

Species

Human