Recombinant Human HGFR/c-MET Protein (aa 25-519, His Tag)

Specifications

Catalog number

PKSH032537-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hepatocyte growth factor receptor (HGF R) is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. Mature HGF R is a disulfide-linked dimer composed of a 50 kDa extracellular α chain and a 145 kDa transmembrane β chain. Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain; consist of secreted extracellular domains; or are deficient in proteolytic separation of the α and β chains. The sema domain; which is formed by both α and β chains of HGF R; mediates both ligand binding and receptor dimerization. HGF stimulation induces HGF R downregulation via internalization and proteasomedependent degradation. Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells.

Abbreviation

HGFR; c-MET

Synonyms

HGF receptor; HGF/SF receptor; Hepatocyte growth factor receptor; MET ; Proto-oncogene c-Met; SF receptor; Scatter factor receptor; Tyrosine-protein kinase Met

UniProt

P08581

Accession Number

P08581

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu25-Gly519

Field of Research

Signal Transduction; Cancer; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

56.9 kDa

Observed Molecular Weight

32&35-57 kDa

Species

Human