Recombinant Human KPNB1 Protein (His Tag)

Specifications

Catalog number

PKSH032583-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Importin subunit beta-1 (KPNB1) is a member of the importin beta family. KPNB1 contains 1 importin N-terminal domain and 19 HEAT repeats. It is involved in nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. Its functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. The import of proteins containing a classical nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits. Each of these subunits is part of the karyopherin family of proteins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. It mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5.

Abbreviation

KPNB1

Synonyms

Importin subunit beta-1; Importin-90; KPNB1; Karyopherin subunit beta-1; NTF97 ; Nuclear factor p97; PTAC97; Pore targeting complex 97 kDa subunit

UniProt

Q14974

Accession Number

Q14974

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Ala876

Field of Research

Signal Transduction; Tags & Cell Markers; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

98.6 kDa

Observed Molecular Weight

90 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, 30% Glycerol, 100mM Nacl, pH 8.0.

Species

Human