Recombinant Human IDO1/IDO Protein (His Tag)

Specifications

Catalog number

PKSH032584-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Indoleamine 2,3-dioxygenase (IDO) is a heme enzyme that initiates the oxidative degradation of the least abundant, essential amino acid, l-tryptophan, along the kynurenine pathway. This protein is normally expressed in the dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. IDO activity is associated with immunosuppression and immune attenuation. Several studies showed that IDO can contribute to immune escape when expressed directly in tumor cells or when expressed in immunosuppressive antigen presenting cells such as tolerogenic dendritic cells or tumor associated macrophages. IDO also is a promising therapeutic target for the treatment of cancer, chronic viral infections, and other diseases characterized by pathological immune suppression.

Abbreviation

IDO1; IDO

Synonyms

3-dioxygenase;3-dioxygenase 1; IDO; IDO-1; IDO1; INDO; Indole 2; Indoleamine 2

UniProt

P14902

Accession Number

P14902

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Gly403

Applications

Enzyme

Field of Research

Signal Transduction; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is 5166. 667 pmol/min/µg pmol/min/μg.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

46.8 kDa

Observed Molecular Weight

40-50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Sodium Acetate, 150mM NaCl, 20% Glycerol, pH 4.5.

Species

Human