Recombinant Human IDE/Insulysin Protein (His Tag)

Specifications

Catalog number

PKSH032593-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Insulin-Degrading Enzyme (IDE) is a secreted enzyme that belongs to the peptidase M16 family. IDE is a large zinc-binding protease and cleaves multiple short polypeptides that vary considerably in sequence. IDE plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. IDE degrades amyloid formed by APP and IAPP. IDE may participate in the degradation and clearance of naturally secreted amyloid β-protein by neurons and microglia. IDE, which migrates at 110 kDa during gel electrophoresis under denaturing conditions, has since been shown to have additional substrates, including the signaling peptides glucagon, TGF α and β-endorphin.

Abbreviation

IDE; Insulysin

Synonyms

Abeta-Degrading Protease; IDE; Insulin Protease; Insulin-Degrading Enzyme; Insulinase; Insulysin

UniProt

P14735

Accession Number

P14735

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met42-Leu1019

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Neuroscience; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

114.3 kDa

Observed Molecular Weight

120 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05% Brij35, 10% Glycerol, pH 7.5.

Species

Human