Recombinant Human IL-17A&IL-17F Heterodimer Protein (His Tag)

Specifications

Catalog number

PKSH032619-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The IL-17 family include IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F. The family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. All members of the IL-17 family have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, yet they have no sequence similarity to any other known cytokines. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes.

Abbreviation

IL-17A&IL-17F Heterodimer

Synonyms

Interleukin-17A; IL-17; IL-17A; IL17A; IL17

UniProt

Q16552; Q96PD4

Accession Number

Q16552&AAH70124.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gly24-Ala155&Arg31-Gln163

Applications

BLI

Field of Research

Cardiovascular; Cancer; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Biotinylated Human IL-17RA-His-Avi on SA Biosensor, can bind Human IL-17A&17F-His with an affinity constant of 8.6 pM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.1&16 kDa

Observed Molecular Weight

15-25 kDa

Species

Human