Recombinant Human Interleukin-18/IL-18 Protein (His Tag)

Specifications

Catalog number

PKSH032625-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-18 is a secreted protein and it belongs to the IL-1 family. IL-18 is a proinflammatory cytokine and produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL-4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. After stimulation with IL-18; natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells.

Abbreviation

Interleukin-18, IL-18

Synonyms

MGC; IL1F; IL-1F; IL18; IGIF; IL-18; IL-1g; IL1F4; GIF; Iboctadekin; IFN-gamma-inducing factor; IL-1 gamma; IL-1F4; Interferon gamma-inducing factor; Interferon-gamma-inducing factor; Interleukin 18; Interleukin-1 gamma; Interleukin-18; MGC12320; IFN gamma inducing factor; IL 1 gamma; IL 18; IL 1g; IL1 gamma; IL18 protein; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interleukin 1 gamma; Interleukin 18 (interferon-gamma-inducing factor) ; Interleukin18

UniProt

Q14116

Accession Number

Q14116

Expression System

HEK293 Cells

Tag

C-His

Sequence

Tyr37-Asp193

Field of Research

Cancer; immunology; metabolism

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.3 kDa

Observed Molecular Weight

16 kDa

Formulation

Lyophilized from a 0.2 µm filtered solution of 20mM PB，150mM NaCl，pH7.4.<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.

Species

Human